|
KMID : 1146920230530020307
|
|
Journal of Pharmaceutical Investigation
2023 Volume.53 No. 2 p.307 ~ p.321
|
|
|
Polyamide 6/tallow modified clay nanofibrous mat coupled with hydrogels for potential topical/transdermal delivery of doxycycline hydrochloride
|
|
Amira Nagy Shehab-ElDin
Rokaya Aly Sobh
Abdelgawad Mohamed Rabie
Wael Sabry Mohamed
Hanaa Elsayed Nasr
|
|
|
Abstract
|
|
|
Purpose : Current innovations in pharmaceutical industries involve the design of functionalized materials for drug delivery applications. Hybrid-bilayer patches of electrospun polyamide 6/tallow modified clay nanocomposite fibers coupled with hydrogels comprising sodium alginate (NaAlg), polyvinyl alcohol (PVA), or a mixture of both were developed.
Methods : The hydrogel precursors were crosslinked either ionically using a CaCl2 solution or physically through repeated freezing?thawing cycles to avoid toxic crosslinking agents. Doxycycline hydrochloride was loaded either by adsorption or active loading methods. The hybrid bilayer patches were evaluated for their physicochemical and morphological properties. Furthermore, in vitro drug release studies in phosphate-buffered saline (PBS) (pH 7.4) and acetate buffer (pH 5.5) were performed, along with antimicrobial activity and toxicity tests.
Results : An increase in the NaAlg ratio caused a gradual decrease in the gel content while greatly increasing the swelling ability. Porous structures were also produced. The in-vitro drug release profiles and corresponding kinetics were mainly dependent on structural factors. An initial burst release was observed from the CaAlg nanofibers (approximately 61.5%?±?0.68% and 52%?±?1.22% of the drug was released during the first 2 h in PBS and acetate buffer, respectively), whereas a relatively slow release rate was achieved from the nanofibers of the physically crosslinked PVA or PVA/NaAlg. The antimicrobial test confirmed that the loaded drug retained its antimicrobial efficacy. The toxicity test using the “Microtox analyzer model 500” exhibited EC50?≥?100, indicating no toxic effect against the luminescent marine bacteria Vibrio fischeri.
Conclusion : The fabricated functionalized patches showed great potential for topical/transdermal drug delivery.
|
|
|
KEYWORD
|
|
|
PA6 nanofibers, Modified clay, Hydrogel, Bilayer patches, Drug delivery
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|